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22 uvrag  (Proteintech)


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    Proteintech 22 uvrag
    22 Uvrag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+uvrag/Beclin+1+Antibody/pm41662369-237-55-58
    Average 96 stars, based on 1514 article reviews
    22 uvrag - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Disrupted apolipoprotein L1-miR193a axis dedifferentiates podocytes through autophagy blockade in an APOL1 risk milieu
    Article Snippet: Lysates from V- and G0-podocytes were first immunoprecipitated following the addition of 5 μg of anti-APOL1 (Proteintech), 5 μg of anti-ATG14L (Abcam), 5 μg of anti-Rubicon (Sana Cruz Biotechnology), or 5 μg of anti-UVRAG (Proteintech) antibodies. .. Lysates from V- and G0-podocytes were first immunoprecipitated following the addition of 5 μg of anti-APOL1 (Proteintech), 5 μg of anti-ATG14L (Abcam), 5 μg of anti-Rubicon (Sana Cruz Biotechnology), or 5 μg of anti-UVRAG (Proteintech) antibodies. .. The immune complexes were then collected using 25 μl of protein A + G Sepharose beads (GE Health Care, Life Sciences) in RIPA buffer.

    Western Blot:

    Article Title: Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption.
    Article Snippet: .. Antibodies and Reagents: Primary antibodies included anti-LC3 (Proteintech, 14600-1-AP), anti-p62 (GeneTex, GTX100685), anti-PINK1 (Proteintech, 23274-1-AP), anti-Parkin (Proteintech, 14060-1-AP), anti-NDP52 (Proteintech, 12229-1-AP), anti-SH3GLB1 (Proteintech, 15422-1-AP), anti-BNP (GeneTex, GTX100538), anti-cTnT (Proteintech, 26592-1-AP), anti-FIP200 (Proteintech, 17250-1-AP), anti-ULK1 (Proteintech, 29005- 1-AP), anti-ATG13 (GeneTex, GTX85183), anti-Ubiquitin (Proteintech, 10201-2-AP), anti-NDUFA8 (Invitrogen, 43–8800), anti-SDHA (GeneTex, GTX636335), anti-NDUFAF1 (Abcam, ab79826), anti-UQCRH (Proteintech, 12364-1-AP), anti-COX5A (Proteintech, 11448-1-AP), anti-ATP5A (Proteintech, 14676-1-AP), anti-VDAC1 (Proteintech, 55259-1-AP), antiPTGS2 (Proteintech, 66351-1-Ig), anti-p-GSK3B (Proteintech, 14850-1- AP), anti-GSK3B (Proteintech, 22104-1-AP), anti-p-ERK1/2 (Proteintech, 28733-1-AP), anti-ERK1/2 (Proteintech, 11257-1-AP), anti-DDDDK tag (Abcam, ab205606), anti-HA tag (Abcam, ab236632), anti-UVRAG (Proteintech, 29190-1-AP), anti-GFP (Abcam, ab6556), anti-PDI (Proteintech, 66422-1-Ig), anti-Pan-Lac (PTM BIO, PTM-1401), anti-myc (Abcam, ab9106), anti-ATG5 (Genetex, GTX113309), anti-ATG16L (Genetex, GTX110619), anti-Beclin1(Proteintech, 11306-1-AP), anti-VPS34 (Genetex, GTX638372), anti-STX17 (Genetex, GTX130212), anti-Tubulin (Proteintech, 11224-1-AP), anti-IDO1 (Proteintech, 13268-1-AP), anti-KMO (Abcam, ab233529), anti-KYNU (ABclonal, A23962), anti-HAAO (Genetex, GTX85015), anti-QPRT (ABclonal, A14349), anti-NMNAT1 (ABclonal, A6672), and OxPhos rodent WB antibody cocktail (Invitrogen, 45-8099). .. HRP-conjugated goat anti-rabbit and goat anti-mouse recombinant secondary antibodies (H+L) were purchased from Proteintech (RGAR001 and RGAM001, respectively).

    Article Title: Aloe Emodin Alleviates Radiation‐Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption
    Article Snippet: .. Primary antibodies included anti‐LC3 (Proteintech, 14600‐1‐AP), anti‐p62 (GeneTex, GTX100685), anti‐PINK1 (Proteintech, 23274‐1‐AP), anti‐Parkin (Proteintech, 14060‐1‐AP), anti‐NDP52 (Proteintech, 12229‐1‐AP), anti‐SH3GLB1 (Proteintech, 15422‐1‐AP), anti‐BNP (GeneTex, GTX100538), anti‐cTnT (Proteintech, 26592‐1‐AP), anti‐FIP200 (Proteintech, 17250‐1‐AP), anti‐ULK1 (Proteintech, 29005‐1‐AP), anti‐ATG13 (GeneTex, GTX85183), anti‐Ubiquitin (Proteintech, 10201‐2‐AP), anti‐NDUFA8 (Invitrogen, 43–8800), anti‐SDHA (GeneTex, GTX636335), anti‐NDUFAF1 (Abcam, ab79826), anti‐UQCRH (Proteintech, 12364‐1‐AP), anti‐COX5A (Proteintech, 11448‐1‐AP), anti‐ATP5A (Proteintech, 14676‐1‐AP), anti‐VDAC1 (Proteintech, 55259‐1‐AP), anti‐PTGS2 (Proteintech, 66351‐1‐Ig), anti‐p‐GSK3B (Proteintech, 14850‐1‐AP), anti‐GSK3B (Proteintech, 22104‐1‐AP), anti‐p‐ERK1/2 (Proteintech, 28733‐1‐AP), anti‐ERK1/2 (Proteintech, 11257‐1‐AP), anti‐DDDDK tag (Abcam, ab205606), anti‐HA tag (Abcam, ab236632), anti‐UVRAG (Proteintech, 29190‐1‐AP), anti‐GFP (Abcam, ab6556), anti‐PDI (Proteintech, 66422‐1‐Ig), anti‐Pan‐Lac (PTM BIO, PTM‐1401), anti‐myc (Abcam, ab9106), anti‐ATG5 (Genetex, GTX113309), anti‐ATG16L (Genetex, GTX110619), anti‐Beclin1(Proteintech, 11306‐1‐AP), anti‐VPS34 (Genetex, GTX638372), anti‐STX17 (Genetex, GTX130212), anti‐Tubulin (Proteintech, 11224‐1‐AP), anti‐IDO1 (Proteintech, 13268‐1‐AP), anti‐KMO (Abcam, ab233529), anti‐KYNU (ABclonal, A23962), anti‐HAAO (Genetex, GTX85015), anti‐QPRT (ABclonal, A14349), anti‐NMNAT1 (ABclonal, A6672), and OxPhos rodent WB antibody cocktail (Invitrogen, 45‐8099). .. HRP‐conjugated goat anti‐rabbit and goat anti‐mouse recombinant secondary antibodies (H+L) were purchased from Proteintech (RGAR001 and RGAM001, respectively).

    other:

    Article Title: Disrupted apolipoprotein L1-miR193a axis dedifferentiates podocytes through autophagy blockade in an APOL1 risk milieu
    Article Snippet: The immune complexes were then collected using 25 μl of protein A + G Sepharose beads (GE Health Care, Life Sciences) in RIPA buffer.

    Incubation:

    Article Title: 5′-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activation
    Article Snippet: .. The samples were then incubated overnight at 4°C with the following primary antibodies: anti-LC3 (#14600-1-AP; Proteintech), anti-GAPDH (#110494-1-AP; Proteintech), anti-EWSR1 (#55191-1-AP; Proteintech), anti-DGCR8 (#60084-1-Ig; Proteintech), anti-Drosha (#55001-1-AP; Proteintech), anti-UVRAG (#29190-1-AP; Proteintech), anti-Flag (#20543-1-AP; Proteintech), and anti-p62/SQSTM1 (#84826-1-RR; Proteintech), each diluted according to the manufacturer’s recommendations. .. After three washes in TBST, the membranes were incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies ( e . g ., #7074 for anti-rabbit or #7076 for anti-mouse; Cell Signaling Technology) at room temperature for 1 h. Finally, the protein bands were visualized using an enhanced chemiluminescence (ECL) detection reagent (#P0018S; Beyotime) and imaged with an Amersham Imager 680 System.

    Article Title: 5'-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activation.
    Article Snippet: .. The samples were then incubated overnight at 4°C with the following primary antibodies: anti-LC3 (#14600-1-AP; Proteintech), anti-GAPDH (#110494-1-AP; Proteintech), anti-EWSR1 (#55191-1-AP; Proteintech), anti-DGCR8 (#60084-1-Ig; Proteintech), anti-Drosha (#55001-1-AP; Proteintech), anti-UVRAG (#29190-1-AP; Proteintech), anti-Flag (#20543- 1-AP; Proteintech), and anti-p62/SQSTM1 (#84826-1-RR; Proteintech), each diluted according to the manufacturer’s recommendations. .. After three washes in TBST, the membranes were incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (e.g., #7074 for anti-rabbit or #7076 for antimouse; Cell Signaling Technology) at room temperature for 1 h. Finally, the protein bands were visualized using an enhanced chemiluminescence (ECL) detection reagent (#P0018S; Beyotime) and imaged with an Amersham Imager 680 System.

    Control:

    Article Title: The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function.
    Article Snippet: Macroautophagy/autophagy is a catabolic process crucial for degrading cytosolic components and damaged organelles to maintain cellular homeostasis, enabling cells to survive in extreme extracellular environments.. ENAH/MENA, a member of the Ena/VASP protein family, functions as a highly efficient actin elongation factor.. In this study, our objective was to explore the role of ENAH in the autophagy process.



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    rabbit anti uvrag  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit anti uvrag
    (A) The expression levels <t>of</t> <t>LC3B</t> and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with <t>UVRAG</t> and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .
    Rabbit Anti Uvrag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+uvrag/UVRAG+Rabbit+mAb/pm40543506-311-190-193
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    rabbit anti uvrag - by Bioz Stars, 2026-10
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    (A) The expression levels of LC3B and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with UVRAG and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .

    Journal: PLOS Biology

    Article Title: CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8 + T cell-mediated killing by inhibiting autophagy

    doi: 10.1371/journal.pbio.3003647

    Figure Lengend Snippet: (A) The expression levels of LC3B and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with UVRAG and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .

    Article Snippet: Antibodies used included primary antibodies against CUL5 (Abclonal, A5369), β-Actin (Proteintech, 66009-1-Ig), PRMT5 (Proteintech, 18436-1-AP), THOC2 (Proteintech, 55178-1-AP), THRAP3 (Proteintech, 19744-1-AP), SNRNP200 (Proteintech, 23875-1-AP), PTBP1 (Proteintech, 12582-1-AP), SF3B1(Proteintech, 27684-1-AP), PRPF8(Proteintech, 11171-1-AP), SF3B2 (Proteintech, 10919-1-AP), HNRNPC (Proteintech, 11760-1-AP), LC3B (Abclonal, A5618), P62 (Proteintech, 18420-1-AP), Rubicon (Proteintech, 21444-1-AP), UVRAG (Proteintech, 29190-1-AP), Beclin1 (Proteintech, 11306-1-AP), HLA-class I (Proteintech, 15240-1-AP), Rabbit control IgG (Abclonal, AC005), Mouse control IgG (Abclonal, AC011), Mouse anti-HA tag (Abclonal, AE008), Rabbit anti-HA tag (Abclonal, AE036), Mouse anti-Flag tag (Abclonal, AE005), and Rabbit anti-Flag tag (Abclonal, AE004); HRP-conjugated secondary goat anti-mouse (Proteintech, SA00001-1), or goat anti-rabbit (Proteintech, SA00001-2) antibodies.

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Cell Culture, CCK-8 Assay, Knockdown, Agarose Gel Electrophoresis, Quantitative RT-PCR, Co-Immunoprecipitation Assay